Leachiane A
Leachianone A
CAS: 97938-31-3
Molecular Formula: C26H30O6
Leachiane A - Names and Identifiers
Leachiane A - Physico-chemical Properties
| Molecular Formula | C26H30O6 |
| Molar Mass | 438.51 |
| Density | 1?+-.0.06 g/cm3(Predicted) |
| Melting Point | 157-158℃ |
| Boling Point | 649.7±55.0 °C(Predicted) |
| Specific Rotation(α) | -43.6 (c, 1.07 in MeOH) |
| pKa | 7.67±0.40(Predicted) |
| Storage Condition | 2-8°C |
| In vitro study | Leachianone A (0-20 μg/ml; 24-72 hours) exhibits a marked inhibition on the survival of HepG2 cells time- and dose-dependently manner, IC 50 values are 6.9 μg/ml, 3.4 μg/ml and 2.8 μg/ml in cells with 24-, 48- and 72-hours treatment, respectively. Leachianone A (10-30 μg/ml; 48 hours) indicates that at low concentration of LA (10 μg/ml), a substantial amount of cells is primarily in the early phase of apoptosis, at higher concentrations, induces a shift of the cell population to late apoptotic/ necrotic stage. Leachianone A (10-30 μg/ml; 48 hours) decreases the precursor of caspase-3 in a dose-dependent manner, reduces the protein level of the pro-forms of upstream initiator caspases, caspases-8 and -9, decreases two downstream substrates, namely inhibitor of caspase-activated DNase(ICAD) and poly-ADP-ribose polymerase (PARP) in HepG2 cells. Cell Viability Assay Cell Line: HepG2 cells Concentration: 0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml, 10 μg/ml, 12 μg/ml, 14 μg/ml, 16 μg/ml, 18 μg/ml, 20 μg/ml Incubation Time: 24-72 hours Result: Inhibited HepG2 cells survival. Apoptosis Analysis Cell Line: HepG2 cells Concentration: 48 hours Incubation Time: 10, 20, and 30 μg/ml Result: Induced the proportion of annexin V-stained cells in both the early and late apoptotic stage. Western Blot Analysis Cell Line: HepG2 cells Concentration: 48 hours Incubation Time: 10, 20, and 30 μg/ml Result: Decreased the protein expression of caspase-3, caspases-8 and -9, reduced ICAD and PARP protein expression. |
| In vivo study | Leachianone A (intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days) significantly diminishes the tumor volume by 17-54% in LA-treated nude mice, when compared with those solely given the vehicle. Animal Model: Male nude mice with human hepatoma HepG2 cells Dosage: 20 mg/kg; 30 mg/kg Administration: Intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days Result: Suppressed the tumor growth in vivo. |
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Product Name: Leachianone A Visit Supplier Webpage Request for quotation
CAS: 97938-31-3
Tel: 18301782025
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Mobile: 18021002903
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CAS: 97938-31-3
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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